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Fig. 4. Gata2 is necessary, and Gata3 is dispensable, for the development of rostral 5-HT neurons. (A,B) Whole-mount immunofluorescence for 5-HT of (A) Gata3^-/+ and (B) Gata3^-/- embryos at E11. (C,D) Whole-mount in situ hybridization for Pet1 on Gata2^-/+ (C), and Pet1 (D, left) and Gata3 (D, top right) on Gata2^-/- (D) embryos at E10.5. A close up of double in situ hybridization for Gata3 (blue) and Nkx2.2 (yellow) in r1 at the ventral midline is shown on the bottom right of D. The floor plate (fp), midbrain (MB), mid-hindbrain boundary (MHB, arrow), 5-HT immunoreactivity (A,B; arrowheads), and the presence (C) versus absence (D) of Pet1 transcript (arrowheads) are marked. (E-H) Immunofluorescence staining on explant cultures from Gata2^-/+ (E,G) versus Gata2^-/- (F,H) embryos, at the equivalent of E13, with antibodies against tyrosine hydroxylase (E,F; TH, red) or Isl1 (G,H, red), compared with 5-HT (E-H, green). (H) In situ hybridization for Gata3 on Gata2^-/- explant cultures (inset, blue).

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