spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. Nkx2.2 and Nkx6.1 mediate Gata expression and 5-HT development in r1. (A,B) Chick hindbrain 3-4 days after electroporation of Nkx2.2, showing whole-mount immunofluorescence for 5-HT (A, red) and in situ hybridization (B) divided into r1 versus r2-3 for Gata3 (purple) and Nkx6.1 (orange). Ectopic (ect.) versus endogenous neurons (en.) are bracketed and rhombomere 1 (r1) is marked (A). Black arrowheads delineate the extent of Gata3 to Nkx6.1 expression on the electroporated (ect) versus control side (B, top). Orange arrowheads indicate the gradient of Nkx6.1 expression, whereas purple and orange arrowheads indicate the ectopic expression (ect. bracketed) of Nkx6.1 and Gata3, respectively (B, bottom). (C) Immunofluorescence on thin (25 µm) sections through r1 on the side of the neural tube electroporated with Nkx2.2 or Nkx6.1 (red), Gata2, (green) and 5-HT (blue), showing the higher expression of Nkx6.1 (arrows) where endogenous and ectopic (bracketed) Gata2 expression and 5-HT neurons develop. (D-F) Immunofluorescence on thin (25 µm) sections through r1 showing endogenous expression of Nkx6.1 (D-F, red), Gata2 (D,F, green), Nkx2.2 (E, inset green) and 5-HT (E,F, blue) at HH20 (D,E) and HH25 (F), with the higher Nkx6.1 expressing ventral region marked by white arrows. (D, inset) Co-localization between high Nkx6.1 and Gata2 in bracketed region. (E, inset) Overlay of Nkx2.2 with the Nkx6.1 and 5-HT expression shown in the main panel. (G,H) Whole-mount immunofluorescence for 5-HT (G, red), or in situ hybridization for Gata3 (purple) and Nkx6.1 (orange) (H), on chick hindbrain performed 3-4 days after electroporation of Nkx2.2 and Nkx6.1. Ectopic (ect.) versus endogenous neurons (en.) are bracketed (G); black arrowheads delineate the extent of Gata3 to Nkx6.1 expression on the electroporated (right) versus control side in (H). (I) Immunofluorescence on thin (25 µm) sections through r1 on the electroporated side of the neural tube, with antibodies against Gata2 (green) and 5-HT (blue). The approximate boundaries of the endogenous high expressing Nkx6.1 domain are marked by arrows, and the ectopic Gata2 and 5-HT positive neurons are bracketed. The midbrain (MB) and mid-hindbrain boundary (MHB, arrow) are indicated on wholemounts; the floor plate (fp) is indicated on sections.





Right arrow Return to article