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Fig. 2. Hyperinnervation of the skin in postnatal and adult K14-noggin animals.
Innervation of the epidermis and upper dermis at comparable locations in the
intervibrissal fur (A-D) and at the mouth of vibrissal follicles (E,F) in
wild-type and K14-noggin overexpressing mice (K14-Nog) as shown by
double-label immunofluorescence with anti-CGRP revealed with Cy3 and anti-PGP
revealed with Cy2. e, epidermis; f, hair follicle; *, the mouth of
a whisker follicle. The broken line indicates the border between the epidermis
and dermis. Green arrowheads indicate examples of FNEs in the epidermis that
are labeled only with anti-PGP. Yellow arrowheads indicate examples of
epidermal FNEs that also label with anti-CGRP. Note that the epidermis of the
K14-Nog mice contains far more FNEs with and without CGRP-IR. Broad arrowheads
with numbers 2, 3 and 4 indicate regions of the second, third and fourth tiers
of the dermal plexus respectively. In K14-Nog mice, the bundles of axons that
compose the fourth tier are much larger and occupy a greater proportion of the
epidermal-dermal border. In wild type, axons with CGRP-IR (yellow arrows) are
mostly restricted to the second and third tiers. In K14-Nog, numerous axons
with CGRP-IR are also located in the fourth tier. However, at E15 there are no
detectable differences in PGP immunostaining between transgenic and control
mice (G,H). Panels A-H are all mice from an Fvb background. (I-L)
Quantification of the innervation to the epidermis and upper dermis in K14-Nog
mice. The quantitative analyses were carried out on five sections for each
mouse and the mean for each parameter obtained. Mean±s.e.m. of three
mice for each genotype are shown (*P<0.05;
**P<0.005; ***P<0.0005).
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