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Fig. 4. In situ hybridization for IGF2 and IGFBP4 expression. Wild-type (A,B,C) and PAPPA-/- (D,E,F) E12.5 embryos hybridized with digoxigenin-labeled antisense RNA probes for IGF2 (A,B,D,E) or IGFBP4 (C,F). (B,C,E,F) Dorsal views, anterior is to the upper right. Strong expression is seen lateral to the neural tube (nt), most probably in the spinal ganglia, as well as in the forelimbs (fl), hindlimbs (hl) and tail (t).





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