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Fig. 2. Identification of rhombomeres in the lamprey. (A) LjKrox20
expression (blue) in relation to the localization of acetylated tubulin
(brown) in a stage 25 embryo (anterior is towards the left). (B) Cranial nerve
roots (arrowheads) and rhombomere boundaries (arrows) are visualized with the
anti-acetylated tubulin antibody. Note that LjKrox20 expression
domain corresponds well to the morphological rhombomere boundaries (compare
arrows in A and B). (C,D) LjPax6 expression (blue) and reticulospinal
neuron (brown) localization. (C) Dorsal view of a stage 26 embryo. Reticular
neurons including I1, B and mir have developed lateral to the LjPax6
expression domain at the hindbrain midline. (D) Lateral view of the same
embryo. Mth is located in r4, where LjPax6 is expressed at low
levels. B, bulbar neurons; I, isthmic reticular neurons; MHB, mid-hindbrain
boundary; mir, medial inferior reticulospinal neurons; Mth, Mauthner neuron;
Mth', auxiliary Mauthner neuron; V1, profundus ganglion; V2/3,
trigeminal ganglion; VII, facial ganglion
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