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Fig. 4. Expression of LjEphC and spatial patterning of reticulospinal neurons. (A,B) Expression of LjEphC in a stage 25 lamprey embryo. (A) Lateral view. LjEphC is expressed in the hindbrain with two clear domains corresponding to r3 and r5. (B) Dorsal view of the same embryo. Note that the otic vesicle is adjacent to an LjEphC-negative hindbrain region, corresponding to r4 (arrows). (C-F) Positions of bulbar (B) and Mth neurons in relation to the expression of LjEphC. Reticular neurons are visualized by whole-mount in situ hybridization with a neurofilament protein antisense riboprobe. (C) Lateral view of a stage 25 embryo. (D) Dorsal view of the same embryo as in C. (E,F) Higher magnification of the embryo in C. (E) Lateral view. (F) Dorsal view. Note that the B and Mth neurons are located in the LjEphC-negative domain corresponding to r4.





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