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Fig. 1. The onset of SerT expression is correlated with axon guidance across the midline. (A,B) Staining for serotonin uptake in red and ß-galactosidase in green (UAS-tau-lacZ driven by egGal4). egGal4 expression of lacZ is seen in all four progeny of NB 7-3, which includes the serotonergic neurons, two other neurons and one pair of lateral neurons that arise from a different lineage. (A) SerT activity was never seen before growth cones crossed the midline in stage 14 ventral nerve cords (10:20-11:20 hours development), but (B) always seen after axons reached the contralateral neuropil at late stage 15 (11:20-13:00 hours). (C) In situ hybridization for SerT mRNA shows robust expression by late stage 15. Staining for the axon scaffold of the ventral nerve cord is shown in brown with mAb BP102. Scale bar: 10 µm.





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