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Fig. 5. robo2 mutants lose eagle expression. Stage 16 wild-type
(A), egMz360 hypomorph (B), robo2
loss-of-function (C) and (EP2582/EP2582;
egMz360/egMz360) (D) nerve cords.
Serotonin uptake, red; Eg, green; yellow, co-localization. M indicates the
position of the ventral midline. (A) The wild-type nerve cord has two
serotonergic neurons per hemisegment, both positive for SerT activity and for
the transcription factor Eagle. (B,C) An egMz360 and a
robo2 mutant show a loss of SerT activity in a percentage of
hemisegments. In the egMz360 (B) CNS, all Eg staining is
absent although some SerT remains, while in the robo2 mutant (C), Eg
is lost only in those cells that lack SerT activity. (D) When two copies of
robo2 (EP2582, Robo gain of function++) are overexpressed in an
egMz360 (hypomorph) background, both Eg expression and
SerT activity are rescued despite the fact that expression of robo2
prevents axons from crossing the midline. Scale bar: 5 µm.
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