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Fig. 6. Overexpression of Robo2 rescues an eagle hypomorphic mutation and
overexpression of Eg rescues a robo2 loss of function. (A) Wild-type
stage 16 nerve cord stained for serotonin uptake showing two serotonergic cell
bodies per hemisegment with axons extending across the midline. (B) Embryos
homozygous for the hypomorphic egMz360 allele (also an
eagleGal4) show 
30% loss of SerT-positive cells as well as axon
guidance defects. (C) Expression of one copy of Robo2 (EP2582, Robo gain of
function) with one copy of egGal4 (heterozygous
egMz360) prevents axons from crossing the midline but does
not disrupt SerT activity. (D) Expression of Robo2 with two copies of the
hypomorphic egGal4 allele (homozygous egMz360)
disrupts axon guidance, but also causes a loss of SerT as seen in B. (E)
However, expression of two copies of Robo2 (Robo2 gain of function++) with two
copies of egGal4 (homozygous egMz360) rescues the
loss of SerT phenotype. (F) Conversely, expression of Eg (egGal4)
rescues both SerT activity and the serotonergic axon guidance phenotype
observed in robo2 mutants (compare with
Fig. 4B). Scale bar: 10
µm.
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