|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. SBRN3 mediates transcriptional activation by Brn3b in HEK 293 cells. On the
left are schematics of the reporter constructs used in the transfection assay.
A contains the minimal rat prolactin promoter (-36prl) alone (P, green box)
upstream of the luciferase cDNA (Luc); B shows the conserved region (yellow
box) in the first intron of mouse Shh sequence cloned upstream of
-36prl; C is the same as B except that the SBRN3 site is mutated (indicated by
a filled black circle); D contains three copies of the consensus Brn3-binding
site (blue boxes) upstream of -36prl. On the right is the normalized
luciferase activity (in arbitrary units) generated from the reporter
constructs co-transfected into HEK 293 cells, with (+) or without (-) the
Brn3b expression vector. Each bar represents the average of three replicate
experiments. Fold change in luciferase activity for each construct in the
presence of Brn3b is indicated at the top of the respective bars.
|
