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Fig. 2. Expression of p53, p63 and p73 proteins in oligodendrocyte lineage cells.
Purified P7 rat OPCs were cultured in PDGF without TH and RA for 10 days. They
were then cultured for 3 or 5 days in either PDGF alone or PDGF and TH to
induce differentiation. (A-C) After 3 days, the cells were fixed and stained
by immunofluorescence for p53 (A), p63 (B) or p73 (C). (D) Cells in PDGF and
TH for 3 or 5 days were fixed and double-stained for both GC and p73. In all
cases, nuclei were counterstained with bisbenzamide. Scale bar: 20 µm.
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