|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Effect of the expression of a wild-type or two dominant-negative forms of
p53 transgenes on OPC differentiation. Purified P7 rat OPCs were
cultured for 2 days in PDGF without TH and RA. They were then infected for 3
hours with either the pBird control vector encoding GFP alone or a vector
encoding GFP and either wild-type (pBird-p53) or a dominant-negative p53
(pBird-DNp53 or pBird-DDp53). After a further day in PDGF alone, the cells
were either left in PDGF alone or switched to PDGF and TH, PDGF and RA, or
medium without PDGF for a further 3 days to induce differentiation. The
percentage of GFP+ cells that had acquired the characteristic
morphology of oligodendrocytes was scored in an inverted fluorescence
microscope. Similar results were obtained when oligodendrocytes were
identified by staining with anti-GC antibody (not shown). In this and the
following three Figures, the results are shown as mean±s.d. of at least
three experiments.
|
