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Fig. 4. Expression of Bapx1 in the mouse middle ear. (A) Expression of
Bapx1 during middle ear development can first be detected at E10.5,
with expression of the gene in a discreet domain in the first branchial arch
(blue arrowhead). (B) By E12.5, Bapx1 expression can be detected in
the region of the condensing middle ear ossicles. (C,D) Closer inspection of
this expression pattern reveals Bapx1 expression around Meckel's
cartilage (defined in C by expression of 
-collagen) and in
condensations that correspond to the condensing tympanic ring (yellow
arrowhead) and gonium (black arrowhead). (E-P) Analysis of Bapx1
expression during late embryogenesis through sagittal sections stained with
either Alcian Blue and chlorantine Fast Red (E,G,I,K,M,O) or by 35S
radioactive in situ hybridisation (F,H,J,L,N,P). (F,H,J,L) Dark-field
photographs, Bapx1 expression shown by white silver grains. (N,P)
Bright-field photos, Bapx1 expression shown by black grains. (E-J)
E15.5, (K,L) E14.5, (M-P) E17.5. (E,F) Expression of Bapx1 both
within and between (arrow) the malleus and incus. (G,H) Expression surrounding
the malleus and incus in the incudo-malleal joint region. (I,J) Expression in
the developing gonium (arrow) and around Meckel's cartilage, but not within
the differentiating tympanic ring. (K,L) Expression under the EAM (arrow).
(M,N) Expression around the developing gonium (arrow) and tympanic. (O,P)
Expression in the incudo-malleal joint (arrow). Me, Meckel's cartilage; M,
malleus; I, incus; S, stapes; Ty, tympanic ring; G, gonium; EAM, external
auditory (acoustic) meatus.
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