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Fig. 4. Ectopic expression of bcd at the posterior pole induces Sxl
expression. Merged images of embryos co-immunostained with
anti-ß-galactosidase (red) and anti-Vasa (green) antibodies. (A) Embryo
produced by wild-type female mated to male carrying the Sxl-Pe:lacZ
reporter transgene. ß-galactosidase is detected in somatic cells but not
in pole cells. (B) Embryo produced by bcd-nos3'UTR
female mated to male carrying the Sxl-Pe:lacZ reporter transgene.
ß-galactosidase is detected in both somatic and pole cells. (C) Embryo
produced by bcd-nos3'UTR female mutant for
tsl mated to male carrying the Sxl-Pe:lacZ reporter
transgene. Expression of the Sxl-Pe:lacZ reporter is no longer
detected in pole cells when tsl function is removed. Embryos shown in
A-C are judged to be female by the activation of Sxl-Pe in somatic
cells.
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