|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. tailless-lacZ reporter is turned on in the newly formed pole cells
of embryos maternally compromised for pgc activity. Wild-type females
or females carrying two copies of the antisense pgc transgene were
mated to males carrying the tll-lacZ reporter. The two panels at the
top show pgc embryos probed with ß-galactosidase antibody and
visualized by DAB staining. The panel on the left shows a cycle 10 embryo
which has a higher number than normal pole cells. Several of these
pgc pole cells express high levels of ß-galactosidase, while
others have little if any ß-galactosidase. At this stage, wild-type
tll-lacZ embryos probed with ß-galactosidase antibody show no
detectable DAB staining in either the pole cells or the soma. The panel on the
right shows a cycle 10 pgc embryo that has ß-galactosidase
positive `Pole cells' at unusual positions (marked with arrows). Some of these
are in the interior of the embryo, while others are near the surface. The pole
cells shown in the middle and lower panels were co-immunostained with
anti-Vasa (green) and anti-ß-galactosidase antibodies (red). All images
show embryos at cycle 10. Note that some of the `pole cells' in the
pgc embryo in the bottom panels express very high levels of
ß-galactosidase but have little or no Vasa protein. Note also that some
of the Vasa-positive pole cells are in the interior of the embryo rather than
at the surface at the posterior pole.
|
