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Fig. 7. MAP kinase signaling is elevated in the pole cells compromised for
pgc function. (A,B) Wild-type embryos were stained with anti ERK
antibodies and signal was visualized with DAB. (A) A syncytial blastoderm
embryo displays increased levels of staining at both the termini. Very little,
if any, signal is detected in the pole cells. (B) Signal specific for anti-ERK
antibodies completely disappears by cellular blastoderm stage. (C,D)
Pre-syncytial cycle 10 embryos co-immunostained with monoclonal anti-ERK
antibody (green) and anti-Vasa antiserum (red). (C',D') The same
embryos as in C and D labeled with only anti-ERK antibody. Pole cells from the
wild-type embryo (C and C') have only a low level of anti-ERK specific
signal whereas pole cells compromised for pgc function (D,D')
have much higher levels.
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