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Fig. 4. Time-course analysis of the pro-1(na48) Pro phenotype. (A)
Synchronized DAPI-stained animals (see Materials and methods) were scored for
the presence of distal mitotic, transition, pachytene, gametogenic and
proximal mitotic nuclei at indicated time points. Data are percent of gonad
arms containing the indicated nuclei at a given time point. For each time
point (46-70 hours at 2-hour intervals), n=62, 30, 66, 42, 58, 65,
38, 71, 60, 53, 80, 57 and 81 gonad arms, respectively. (B F) One
characteristic gonad arm from selected time points. (B) 52 hours:
all-proliferative; (C) 58 hours: distal mitosis, transition and proximal
mitosis; (D) higher-magnification view (different focal plane) of C to show
transition nuclei (left) distal to mitotic nuclei (right); (E) 64 hours:
distal mitotic, transition, pachytene and proximal mitotic nuclei; (F) 66
hours: gametogenesis just distal to proximal mitosis. Arrowheads indicate the
abnormal gametogenesis/proximal mitosis border. Asterisks indicate the distal
end of the gonad arms. Scale bars: 10 µm in D; 25 µm in B,C,E,F.
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