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Fig. 1. (A) Alignment of the amino acid residues of the central region of Ci-ZicL
with those of Cs-ZicL. The five zinc finger motifs are boxed, and conserved
CCHH are indicated by asterisks. This region was used to produce a
GST/ZicL(ZF) fusion protein. (B-D) Zygotic expression of Ci-ZicL, as
revealed by whole-mount in-situ hybridization. (B) A 32-cell, (C) a 64-cell
and (D) a 110-cell stage embryo, vegetal view. (E,F) The effect of suppression
of Ci-ZicL function on expression of Ci-Bra, in (E) a
control and (F) a Ci-ZicL-suppressed embyro at the 110-cell stage.
(G-J) Schematic representations of the expression of FoxD, ZicL,
Snail and Brachyury genes. Embryos at (G) the 16-cell, (H)
32-cell, (I) 64-cell and (J) tailbud stage, viewed from (G-I) the vegetal pole
and (J) the lateral side. Blastomeres are named according to the nomenclature
of Conklin (1905). Blastomeres
whose names are colored green in (H) and (I) are those that give rise to
notochord cells.
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