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Fig. 5. Misexpression of Ci-ZicL promotes ectopic lacZ expression in blastomeres of non-notochord lineages, while suppression of Ci-ZicL does not activate lacZ expression. (A) p(-3.5k)Ci-Bra/lacZ and Ci-ZicL mRNA, (B) p(-3.5k)Ci-Bra/lacZ with ZicL-b1/ZicL-b2 double-mutation and Ci-ZicL mRNA, or (C) p(-3.5k)Ci-Bra/lacZ and Ci-ZicL morpholino antisense oligonucleotides were injected into fertilized eggs, and the reporter expression was examined at the 110-cell stage by whole-mount in-situ hybridization. (D,E) p(-3.5k)Ci-Bra/lacZ, (F) p(-3.5k)Ci-Bra/lacZ and Ci-ZicL mRNA, or (G) p(-3.5k)Ci-Bra/lacZ with ZicL-b1/ZicL-b2 double-mutation and Ci-ZicL mRNA were injected into fertilized eggs, embryos were allowed to develop to the 110-cell stage and cleavage was arrested for about 6 hours (equivalent to the early tailbud stage), and then the reporter gene expression was examined by histochemical detection of ß-galactosidase activity.





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