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Fig. 1. FGF2 induces OLPs through FGFR1. (A) Dissociated cells from mouse E13.5
neocortex (boxed region) were cultured in defined medium in the presence or
absence of different FGFs for 3 days in vitro (DIV). (B-D) The cultures were
fixed and immunolabelled with polyclonal anti-NG2. Control cultures lacked NG2
immunoreactivity (B). Numerous NG2-positive cells developed in 10 ng/ml FGF2
(C) and 50 ng/ml FGF2 (D). (E) The numbers of OLPs induced by FGF8, 9 or 10
were at least 100x lower than with FGF2, suggesting that the inducing
activity is mediated via FGFR1. NG2-positive cells were counted in more than
10 randomly selected fields on each of two coverslips (x63 microscope
objective). At least two independent experiments gave similar results, one of
which is illustrated. (F) Dorsal spinal cord explants from chick
Hamilton-Hamburger stage12 (E2) developed O4-positive OLPs when cultured in
the presence of FGF2, even in the presence of cyclopamine (FGF2 + cyc). The
number of O4-positive explants and the total number of explants are shown
above each bar.
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