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Fig. 2. Induction of OLPs by the Hedgehog agonist SHHAg1.2.Mouse E13.5 neocortical
neuroepithelial cells were cultured in the presence or absence of SHHAg for 4
DIV. The cultures were fixed and immunolabelled with polyclonal anti-NG2. (A)
Control cultures lacked NG2 immunoreactivity. (B) Numerous NG2-positive cells
developed in the presence of 100 nM SHHAg1.2. (C) The dose-response curve
shows induction of NG2-positive cells at a half-maximal concentration of
SHHAg1.2 of 
25 nM.
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