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Fig. 3. Rapid induction of OLIG2 by SHHAg or FGF2. Neocortical precursors from E13.5 mice were cultured in the presence or absence of FGF2 or SHHAg and assayed for OLIG2 immunoreactivity at different times. OLIG2-positive nuclei appeared within the first 20 hours in both FGF2-treated (C,F) and SHHAg-treated (B,E) cultures. The experiment was quantified (G) by counting OLIG2-positive cells in more than 10 randomly selected fields on each of two separate coverslips (x63 microscope objective) and are displayed as mean±s.d.





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