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Fig. 3. Rapid induction of OLIG2 by SHHAg or FGF2. Neocortical precursors from
E13.5 mice were cultured in the presence or absence of FGF2 or SHHAg and
assayed for OLIG2 immunoreactivity at different times. OLIG2-positive nuclei
appeared within the first 20 hours in both FGF2-treated (C,F) and
SHHAg-treated (B,E) cultures. The experiment was quantified (G) by counting
OLIG2-positive cells in more than 10 randomly selected fields on each of two
separate coverslips (x63 microscope objective) and are displayed as
mean±s.d.