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Fig. 8. Induction of OLPs in ventral spinal cord or ventral forebrain cultures is
independent of FGFR-TK activity. (A) Ventral spinal cord explants from
Hamilton and Hamburger stage 12 (E2) chicks developed O4-positive OLPs when
cultured without exogenously added growth factors. Their development was
strongly inhibited by cyclopamine but only weakly by PD173074. FGF2 in the
absence of complementary Shh activity (i.e. in the presence of cyclopamine)
was unable to induce O4-positive OLPs. (B-E) Dissociated cells from mouse
E10.5 ventral forebrain (boxed region) were cultured in the presence of the
cyclopamine or PD173074 for 10 days, then the cultures were fixed and
immunolabelled with anti-NG2. Control cultures developed numerous NG2-positive
cells (C). Their production was inhibited by cyclopamine (D) but not by
PD173074 (E). (F) The experiment was quantified by counting NG2-positive cells
in more than 10 randomly selected fields on each of two separate coverslips
(x63 objective) and the results displayed as mean±s.d. Similar
data were obtained in at least two independent experiments.
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