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Fig. 4. Canonical Wnt/ß-catenin signaling is required in neural crest
precursors cell-autonomously. (A) As a control, rhodamine dextran-labeled
wild-type neural crest precursors (red) were transplanted into a wild-type
host embryo. The transplanted cells express Foxd3, detected with anti-Foxd3
antibody (green) when transplanted into a wild-type host embryo. Inset is a
single confocal slice to indicate co-localization in single transplanted cell
(white arrowhead). (B,C) Transgenic crest precursors fail to express Foxd3
when transplanted into a wild-type environment. Embryo shown is a dorsal view
with anterior to the top. Inset in (B) is magnified in (C). (D-F) Wild-type
cells express Foxd3 when transplanted into a transgenic environment. (D)
Foxd3-positive nuclei, detected with anti-Foxd3 primary antibody (red), are
observed in transplanted cells from wild-type donor embryos. (E) GFP-positive
nuclei (green) are observed in the 
Tcf background after heat-activation
of the transgene. (F) Stacked confocal images have been merged to show of
failure of Foxd3-positive nuclei to co-localize with background GFP
expression.
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