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Fig. 4. Lack of primary spongiosa in Cre10; Smon/c embryos. (A-D) H&E staining of longitudinal sections through the tibia at P3. The boxed regions in A and B are shown at a higher magnification in C and D. The straight lines in C and D define a region of the same length that in the wild type (C) but not in the mutant (D) is populated by cells with morphology that is distinct from that of the marrow cells. (E-H) In situ hybridization using 35S labeled riboprobes for osteocalcin (E,F) and TRAP (G,H) on longitudinal sections of the tibia at P3. Signals are shown in red and the sections are counterstained with Toluidine Blue.





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