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Fig. 5. Analysis of Hh response and Ihh expression in skeletal elements. In situ
hybridization using 35S-labeled riboprobes for Ihh (A,B)
and Ptc1 (CE) on longitudinal sections of the tibia at P3. Signals
are shown in red and the sections are counterstained with Toluidine Blue. The
boxed region in C is shown at a higher magnification in E. The straight lines
in E demarcate the region populated by cells with morphology that is
distinct from that of the marrow cells, similar to that defined in C and D of
Fig. 4.
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