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Fig. 3. Math1 and Mash1 are transcriptional activators in their neurogenic and
neuronal cell-type-specific activities. (A-L) HH14-16 chick neural tubes were
electroporated in ovo with expression constructs VP16 (A,B), Math1bHLH-VP16
(C,D), Mash1bHLH-VP16 (E,F), EnR (G,H), Math1bHLH-EnR (I,J) and Mash1bHLH-EnR
(K,L), harvested at 24 hours. (A,C,E,G,I,K) Immunofluorescence using anti-myc
antibodies to assay for expression of the transgene demonstrates the movement
of the cells to the lateral neural tube where differentiating neurons reside.
White dots outline the injected side of the neural tube and arrows indicate
the lateral (C,E) or medial (I,K) position of the electroporated cells within
the neural tube. (M) Quantification of these data shown as a ratio of
fluorescence intensity (FI) in the lateral half versus the medial half of the
neural tube (M). (B,D,F,H,J,L) Interneuron populations dI1 and dI3 were
detected using anti-Lhx2/9 (red) or Islet1 (green). (N) Quantification of
these data shown as a ratio of the number of labeled cells on the
electroporated side (right, indicated by arrowhead) versus the number of
labeled cells on the control side (left). VP16 and EnR on their own have no
effect in these assays. Math1bHLH-VP16 and Mash1bHLH-VP16 approximate the
activity of full-length Math1 and Mash1 in both the neuronal differentiation
and cell-type specification activities. In contrast, Math1bHLH-EnR and
Mash1bHLH-EnR have the opposite effect. **P<0.001.
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