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Fig. 5. Essential structural components of Math1 and Mash1 required for their
neuronal specification properties are identified. (A) Schematic of the Math1
and Mash1 chimeric bHLH domains used to identify amino acids required for
their neuronal specification properties. Math1 sequences are shown in green
and Mash1 sequences are in blue. The first six constructs are in the context
of Math1 with the domain replaced by Mash1 sequences shown in parentheses. The
next six constructs are in the context of Mash1 with the domain replaced by
Math1 sequences shown in parentheses. Math1 HLH (green) and Mash1 HLH (blue)
were also tested in the context of MyoD (red). The effect of each chimeric
protein, in increasing (+) or decreasing (-) dI1 and dI3 interneuron
populations as described in Fig.
2, is shown. (B) Quantification of Lhx2/9- and Islet1-expressing
cells in the electroporated side versus the control side for each construct.
**P<0.001. (C) Double immunofluorescence with Lhx2/9
(red) and Islet1 (green) showing the aberrant co-expression (yellow) of these
HD factors in neural tubes electroporated with Mash1 with the helix 2 of
Math1.
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