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Fig. 3. In vivo comparison of IPL formation and sublamination in wild-type and lak mutants. Time-lapse imaging of retinas in line 220 wild-type and lakritz (lak) mutant backgrounds during IPL formation and differentiation. Both IPL formation and sublamination are delayed in the mutants. Each image is the z-projection of 3 µm confocal image stacks. Dorsal retina is towards the top. All three animals shown here were age-matched siblings, and were imaged concurrently. In all images, arrows indicate location of the developing IPL. Opposing arrows at 82 (wild type) and 85 (lak2) hpf indicate location where sublamination is apparent. Note that the IPL of lak2 becomes more organized with time. Movies showing time-lapse recordings from additional wild-type and lak siblings are provided at http://dev.biologists.org/supplemental (Movie 3A,B).





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