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Fig. 4. AC errors during IPL formation in lak mutants. (A,B) Confocal
images of the same region of a lak mutant retina at 55 hpf (A) and 71
hpf (B). GFP+ AC neurites (arrows) initially accumulated near the
internal limiting membrane (ILM; broken line) at various orientations, but
with time, the forming IPL moved away from the ILM. Also shown in A are
examples of GFP+ AC bodies (1) abutting the ILM, a feature commonly
observed at this early stage in the mutants, but not apparent in wild type.
(C,D) A disorganized region of mutant retina at 60 (C) and 66 (D) hpf. (C) AC
neurites (arrow) are oriented abnormally, growing obliquely to the ILM. (D) An
ectopic bundle of AC neurites (arrow) has formed external to the IPL. Some
GFP+ neurites grow directly into this bundle rather than into the
main GFP+ plexus. (E) The lak retina shown in C,D at 70
hpf, demonstrating regions of relatively normal IPL location and orientation
(1); abnormal regions where there are gaps in the GFP+ plexus (2);
neurites oriented perpendicular to the ILM (3); and GFP+ AC somata
agglomerating in a column (4). See Movie 4 at
http://dev.biologists.org/supplemental
for additional views of this retina and others of similar age. (F,G) Examples
of AC neurites in an 82 hpf lak retina linking neighboring cells
(arrows), suggesting the tendency of ACs to grow neurites toward each other
despite disorganization in their cell body locations. See Movie 5, which
illustrates this tendency. All panels depict line 220.
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