|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. The lak gene is not required within ACs for dendrite morphogenesis
or sublaminar targeting. (A-C) Normal morphology and monostratification of
line 220 GFP+ ACs situated in wild-type retinas, as revealed by
transplantation into non-GFP hosts. (A) Wild-type ACs in a wild-type host.
Reconstructions of confocal stacks are shown en face and rotated
40° and 90°. Cell 1 has a symmetrical dendritic field and tightly
packed processes, while Cell 2 has a larger, asymmetric dendritic field (note
in the 90° rotation that the primary dendrite is already projecting to the
left) and sparse, highly branched processes. Both cells are monostratified in
the same IPL sublayer (see 90° rotation). (B) Two more monostratified ACs
from a different wild type into wild type chimera. (C) A large-field
symmetrical AC derived from a lak mutant develops normally in a
wild-type host retina. A 90° rotation shows that its arbors are
monostratified. (D-F) Projection errors of ACs situated in lak mutant
retinas. A wild-type-derived AC in a lak mutant retina (D) projects
diffusely into the IPL, resembling the projection errors (arrows) made by
lak mutant ACs in mutant retinas (E,F). (G-J) Sections through
chimeric retinas, cut perpendicular to the IPL. When the host retina is wild
type (G,H), donor-derived GFP+ AC processes are confined to
specific sublayers (1), regardless of whether the donor is wild type (G) or
lak mutant (H). Wild-type ACs in a lak mutant retina (I)
show local perturbations of sublaminar targeting, similar to the phenotype of
lak-into-lak chimeras (J). Arrow 1, normal stratification;
arrow 2, disrupted stratification.
|
