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Fig. 5. Lamination of the stage 35 retina as shown by transcription factor
immunoreactivity. Representative fluorescence micrographs showing
transcription factor immunoreactivity in the central region of stage 35
retina. (A) Pax6, (B) Prox1, (D) Ap2
, (E) Prox1, (G) Lim3, (H) Prox1,
(J) Lim3, (K) Chx10, (M) Lim1 and (N) Chx10. (C,F,I,L,O) Merged image of the
fluorescence micrographs presented in the top two rows. (P) Schematic summary
of the immunoreactivity shown in A-O. (+) Moderate and (++) strong
immunoreactivity. The results are representative of and were collected from
more than three animals in each case. ONL/onl: outer nuclear layer, INL/inl:
inner nuclear layer (a: outer external INL, b: inner external INL, c: outer
internal INL, d: inner internal INL), GCL/glc: ganglion cell layer. Scale bar:
A (for A-O) 20 µm.