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Fig. 5. Lamination of the stage 35 retina as shown by transcription factor immunoreactivity. Representative fluorescence micrographs showing transcription factor immunoreactivity in the central region of stage 35 retina. (A) Pax6, (B) Prox1, (D) Ap2{alpha}, (E) Prox1, (G) Lim3, (H) Prox1, (J) Lim3, (K) Chx10, (M) Lim1 and (N) Chx10. (C,F,I,L,O) Merged image of the fluorescence micrographs presented in the top two rows. (P) Schematic summary of the immunoreactivity shown in A-O. (+) Moderate and (++) strong immunoreactivity. The results are representative of and were collected from more than three animals in each case. ONL/onl: outer nuclear layer, INL/inl: inner nuclear layer (a: outer external INL, b: inner external INL, c: outer internal INL, d: inner internal INL), GCL/glc: ganglion cell layer. Scale bar: A (for A-O) 20 µm.





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