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Fig. 1. dpp signaling activity is restricted to GSCs and some cystoblasts
where bam transcription is actively repressed. (A) Diagram showing
GSCs, their differentiated progeny and surrounding somatic cells. In panels
B-F, cap cells are highlighted by circles, whereas GSCs are indicated by
asterisks. (B) Tip of the Dad-lacZ germarium labeled for nuclear
ß-Gal (red), Hts (green, fusomes) and DAPI (blue), showing high
Dad expression in GSCs and in a cystoblast (arrow), but not in
another cystoblast (arrowhead). (C) Tip of the bam-GFP germarium
labeled for GFP (green), Hts (red, fusomes) and DAPI (blue), showing
bam expression in a cystoblast (arrowhead) and cysts, but not in
GSCs. (D) Tip of the bam-GFP;Dad-lacZ germarium labeled for
ß-Gal (red), GFP (green) and DAPI (blue), showing that GSCs and two
cystoblasts (arrows) express high Dad but no bam, and that a
cystoblast (arrowhead) has low Dad and begins to express
bam. (E,F) Tip of the bam-GFP germarium labeled for pMad
(red), GFP (green), Hts (blue, fusomes) and DAPI (white, F), showing high pMad
accumulation but no bam expression in GSCs, and low pMad but
bam expression in a cystoblast (arrowhead, E). TF, terminal filament;
GSCs, germline stem cells; SS, spectrosome; Cpc, cap cells; CB, cystoblast;
FS, fusome; IGS, inner sheath cells; CS, cysts. All micrographs are shown at
the same scale. Scale bar: 10 µm.
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