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Fig. 3. dpp overexpression is sufficient for repressing bam
transcription in single germ cells. Germaria in panels A and C-F are labeled
for Hts (red, fusomes), GFP (green) and DAPI (blue), whereas the germarium in
B is labeled for Vasa (red, germ cells) and Hts (green, fusomes). Circles
highlight cap cells and asterisks indicate GSCs. (A) A germarial tip showing
c587-gal4-driven UAS-GFP expression in inner sheath cells
but not in cap cells. (B) A c587-gal4;UAS-dpp germarium resulting
from dpp overexpression is filled with single germ cells with a
spectrosome. The inset shows the tip of the germarium (highlighted by a
rectangle in B) at a higher magnification (4x), containing only germ
cells with a spectrosome (arrows). (C) Tip of the
c587-gal4;bam-GFP;UAS-dpp germarium showing that the accumulated
spectrosome-containing germ cells (two indicated by arrows) a few cells away
from the tip of the germarium fail to express bam-GFP. (D) Middle
portion of the c587-gal4;bam-GFP;UAS-dpp germarium showing that
spectrosome-containing germ cells (two indicated by arrows) fail to express
bam-GFP. (E) Tip of the hs-gal4;UAS-dpp germarium showing no
bam expression in GSCs, but expression in differentiated germ cells
without any heat-shock treatments. (F) Tip of the hs-gal4;UAS-dpp
germarium showing no bam expression in GSCs and in
spectrosome-containing germ cells (two indicated by arrows) distant from the
tip after three days of heat-shock treatments. Scale bars: in A, 10 µm for
A,C-F; in B, 60 µm.
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