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Fig. 6. punt and Med are required cell-autonomously in GSCs to repress bam transcription. Germaria from punt¹⁰⁴⁶⁰/punt¹³⁵ mutant females cultured at 18°C for 2 days (A), or at 29°C for 2 (B) or 7 (C,D) days, are labeled for Hts (red, fusomes and somatic follicle cells), bam-GFP (green) and DAPI (blue). Germaria in E-H from the punt¹⁰⁴⁶⁰/punt¹³⁵ mutant females cultured at 29°C for 4 days are labeled for pMad (red), bam-GFP (green), Hts (blue, fusomes) and DAPI (white). Germaria in I-L are labeled for arm-lacZ (red), GFP (green), Hts (blue) and DAPI (white). F,H,J and L represent corresponding DAPI stainings for E,G,I and K, respectively. Circles highlight cap cells, whereas asterisks indicate GSCs. (A-D) Germarial tips showing two GFP-negative GSCs (A), one GFP-positive and one GFP-negative GSC (B), one GFP-positive GSC (C) and no GSC (D). (E-H) Germarial tips showing two GFP-positive GSCs with severely reduced pMad (E,F) and one GFP-positive GSC with severely reduced pMad (G,H). (IL) Germarial tips showing a bam-GFP-positive marked punt¹³⁵ GSC (outlined by a dashed line; I) and a bam-GFP-positive marked Med²⁶ GSC (dashed line; K). The marked GSCs are identified by loss of arm-lacZ expression. All micrographs are shown at the same scale. Scale bar: 10 µm.

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