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Fig. 6. Overexpression of dpp but not gbb completely represses
bam transcription in the testis. The testes in A and B are labeled
for FasIII (red), Hts (green) and DNA (blue), whereas the testes in C and D
are labeled for GFP (green), Hts (red) and DAPI (blue). The hub cells are
identified by FasIII staining (red) in A and B, and are highlighted by circles
in C and D. (A) The tip of a dpp-overexpressing testis showing more
hub cells and slightly more single germ cells with a spectrosome (arrows) two
or three cells away from the hub cells. (B) The tip of a
gbb-overexpressing testis showing a normal hub and normal germ cell
development. (C) The tip of a dpp-overexpressing testis showing that
late differentiated germ cells (a two-cell cluster indicated by an arrow; a
16-cell cluster indicated by an arrowhead) fail to express bam-GFP.
(D) The tip of a gbb-overexpressing testis showing that
bam-GFP expression in the two-cell clusters (arrows) is delayed but
is expressed in late differentiated germ cells (arrowhead). All the images are
shown at the same magnification. Scale bar: 10 µm.
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