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Fig. 2. Overexpression of ph leads to defects in follicle formation. All
ovarioles are oriented with the anterior towards the top left corner. (A,B,C)
DAPI staining of 4061/+ (A) and 4061/+; da-Gal4/+ (B,C)
ovarioles. (B) Compound follicle with a `curved' aspect to the follicular
epithelium (arrow). (C) The first cysts in the germarium are contained within
a single epithelium, without follicle individualization (arrowhead); arrow
points to apposed follicles. (D) Representation of P{y+}UAS and
PlacW insertions at the ph locus in the P[4061] and
phlac lines, respectively. The P{y+}UAS is
inserted 99 pb upstream of the ATG, in the sense orientation, whereas the
PlacW is inserted in the first intron. (E-H) Control (F) and mosaic
ovarioles (E,G,H) in which ph overexpressing cellular clones are
detected by the expression of a GFP reporter construct
(E',G',H'-H'''). Ovarioles are stained with DAPI
(E,F,G,H), anti-Orb antibodies (E'') and anti-Fas III antibodies
(F',G'',H'-H'''). Compound follicle (E), with a `curved'
epithelium, results from encapsulation of two oocytes as visualized by Orb
staining (E'', arrowheads). (G-G''') Abnormal accumulation of
germline cysts in an entirely clonal germarium (compare G'' and
F'); prefollicular cells that overexpressed ph began to migrate
over germline cysts (G'', asterisks) but failed to completely
individualize them. (H) Compound follicle with two germline cysts encapsulated
together; this follicle contains four groups of polar cells (arrowheads,
H'-H''': three different focal planes of the same follicle).
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