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Fig. 5. polyhomeotic function is required for interfollicular stalk and polar cell differentiation. (A-C) Nuclear DAPI staining reveals the presence of long stalks in phlac mutant ovarioles (B,C, compare to wild-type, A). (A) In wild-type interfollicular stalk cells, {alpha}-Spectrin ({alpha}-Spe) is strongly expressed (A', inset), whereas Fas III (FIII) is almost absent (A'', inset). (B,C) In phlac ovarioles, long interfollicular stalks fail to differentiate correctly as interfollicular stalk cells express both {alpha}-Spe (B', inset) and Fas III (C', inset). (D,E) phlac follicles with fewer (D) or more (E) than 16 germline cells, containing one pair of polar cells at each pole, as revealed after staining with anti-Fas III antibody (D',E'). (F,F') DAPI staining (F) and anti-ß-Gal antibody staining (F') of a compound stage 4 follicle in a phlac/phlac;A101/+ ovary. (G) Percentage of phlac/+; A101/+ (blue) and phlac/phlac; A101/+ (pink) stage 2 to 6 egg chambers containing 0-5 and more polar cells (n>=34 for phlac/+; A101/+ and n>=56 for phlac/phlac; A101/+for each stage). These data concern anterior polar cells.





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