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Fig. 4. PKA catalytic activity is increased in Pka-R118304. PKA
activity in extracts of
Pka-R118304/Df(3L)ri-XT106 adult flies was
measured in the absence or in the presence of 5 µM cAMP. In the absence of
exogenous cAMP, Pka-R118304 mutant extracts show an
approximately 1.2-fold increase in PKA activity over wild type (left panel).
In the presence of saturating concentrations of cAMP, mutant extracts show a
1.5- to 2-fold elevation in PKA activity over the wild type, revealing an
excess of PKA catalytic subunit activity in the
Pka-R118304 mutant (right panel). This is a summary of
assays performed independently three times; bars indicate s.e.m. The
differences in PKA activities between wild type and mutant in the presence or
absence of exogenous cAMP are statistically significant as determined by a
two-sample t-test (P<0.001).
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