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Fig. 2. Overexpression of hfp inhibits cell cycle entry in the developing
eye and wing. (A,B) Scanning electron micrographs of adult eyes of wild type
(A) and GMR-GAL4, UAS-hfp/+; UAS-hfp/+ (B). Scale bar equals 200
µm. (C-F) Eye imaginal discs from wild type (C,E) and
GMR-GAL4,UAS-hfp/+; UAS-hfp/+ (D,F) third instar larvae, co-labelled
with BrdU (C,D) and anti-phosphohistone H3 antibody (E,F). Posterior is to the
left. Yellow bars indicate the MF. (G-J) Adult wings mounted in Canada balsam
(G-I) or fresh (J) from en-GAL4,UAS-GFP (G) and
en-GAL4,UAS-GFP/+,UAS-hfp/+ (H-J) flies. (K-N) Third instar wing
discs from en-GAL4,UAS-GFP (K,M) and
en-GAL4,UAS-GFP/+,UAS-hfp/+ (L,N) flies, co-labelled using GFP
antibody staining to mark the posterior region of the wing disc (K,L) and BrdU
(M,N). The ZNC is marked with an arrow, and in (N) the GFP-positive region is
outlined in white.
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