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Fig. 1. fkh-6 is required for gonadal development. (A) Molecular basis of
fkh-6 mutations. Boxes indicate exons; lines indicate introns. The
forkhead domain (black) is encoded by two exons. Arrows indicate locations of
mutations. The ez16 and q641 alleles are both single base
changes, indicated in red: ez16 affects the ATG start codon and
q641 affects the predicted splice donor site of the second intron.
The q641 transcript is spliced aberrantly, resulting in a two amino
acid insertion in the forkhead domain (see text). (B,C) Adult XO males stained
with DAPI to highlight gonadal nuclei. (B) Wild-type adult male gonad with
elongate, J-shaped gonad. (C) fkh-6(ez16) adult male with
disorganized gonad that has failed to elongate. Sperm are indicated by arrow.
(D-F) Male gonads, differential interference contrast (DIC) optics. (D)
Wild-type adult male with germline (gl), mature sperm, seminal vesicle (sv)
and vas deferens (vd) indicated. (E) fkh-6(ez16) adult male with
vulva indicated (arrowhead). (F) fkh-6(ez16) male at higher
magnification, with mature sperm indicated (arrow). (G) Wild-type young adult
XX hermaphrodite showing posterior gonad arm, with spermatheca (spth), oocytes
(ooc) and embryo (emb) indicated. (H) Young adult fkh-6(ez16)
hermaphrodite; embryo is lodged in spermatheca and fertilized eggs, and
oocytes can be seen backing up behind embryo. (I,J) Adult hermaphrodites
stained with DAPI. (I) Wild-type adult hermaphrodite with two gonad arms
indicated by brackets. (J) fkh-6 adult hermaphrodite showing the
one-armed gonad phenotype that occurs in 
10% of XX mutants. (K,L) Early
L3 wild-type and fkh-6 hermaphrodites at ten-cell somatic gonad
primordium (SPh) stage. Dorsal and ventral uterine precursor cells are
indicated by triangles; larger sheath/spermathecal precursor cells (SS) are
indicated by arrow. (K) Wild-type early L3 hermaphrodite. Three of the six
dorsal and ventral uterine precursor cells are visible, as is one of the four
SS cells (other SPh cells are out of focal plane or obscured by intestine).
(L) fkh-6 early L3 hermaphrodite showing normal position of SPh
cells. Three of six dorsal and ventral uterine precursors are visible,
including differentiating anchor cell (AC). Two of four SS cells are in focal
plane. (M) Wild-type adult male tail, with sensory rays and other copulatory
structures. (N) fkh-6 adult male tail, showing normal morphology. (O)
Ventrolateral view of wild-type adult male showing egl-15::gfp
reporter expression in sex muscles in the tail
(Harfe et al., 1998). (P)
Ventral view of fkh-6 adult male expressing egl-15::gfp in
sex muscles with normal morphology.
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