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Fig. 2. Sex reversal of cell fates in the fkh-6 male gonad. Left column (A,D,G,J,M): wild type XX hermaphrodites. Middle column (B,E,H,K,N): wild type XO males. Right column (C,F,I,L,O): fkh-6(ez16) mutant XO males. All panels are overlaid DIC and fluorescence images, except those showing leIs8, which are DIC images of ß-gal stained animals. For each reporter, fluorescence images are identical exposures for all genotypes. Cell types expressing each reporter in wild-type hermaphrodites are as follows. cdh-3::gfp is expressed in the hermaphrodite anchor cell (AC, arrow), but not in wild-type males. lim-7::gfp is expressed in hermaphrodite sheath cells. leIs8 is a lacZ reporter expressed in hermaphrodite spermathecal cells and some uterine cells. leEx780 is a gfp reporter expressed in spermathecal cells. ezIS1 is a gfp reporter not expressed in hermaphrodites, but expressed in male seminal vesicle and vas deferens (see Materials and methods).

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