DEV01036 Supplemental Data

Files in this Data Supplement:

• Figure S1 -

  Fig. S1. Cardiac development proceeds normally in mice lacking Ccm1. (A,B) Despite the development of extensive vascular defects, endocardial cushion formation is normal at E9.0 (arrows) in mice lacking Ccm1. (C,D) Myocardial trabeculation is present in Ccm1^–/– embryos at E9.5. (E,F) Expression of Hand1 (also known as eHand) in the heart tube of mice lacking Ccm1 is normal at E8.5. (G,H) Expression of Hand2 (also known as dHand) is normal in Ccm1^–/– embryos at E8.5. (I,J) Isolated heart preparations from E8.5 embryos demonstrate normal cardiac looping and similar expression of Nppa (natriuretic precursor peptide, also known as atrial natriuretic factor) between wild-type and Ccm1 mutant embryos. a, atrium; v, ventricle; bc, bulbus cordis; ct, conotruncus. Scale bars: 100 µm.