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Fig. 6. Neural patterning is not perturbed in Ccm1^-/- mice. (A-H) In situ hybridization of Ccm1^+/+ and Ccm1^-/- embryos at E8.5. Arrowheads identify the isthmus between midbrain and hindbrain. (A,B) The expression of the homeobox gene Six3 is restricted to the forebrain. (C,D) The expression of the transcription factor Otx2 remains restricted to the forebrain and midbrain in both Ccm1^+/+ and Ccm1^-/- mice. (E,F) Transcript for Fgf8 is detected from the isthmus, bounded by the midbrain and hindbrain (arrowheads in all panels). (G,H) The homeobox gene Gbx2 is expressed throughout the hindbrain, up to the junction with the midbrain (or isthmus) in both wild-type and Ccm1 homozygous mutant embryos. (I,J) The expression of the leucine zipper transcription factor MafB is restricted to rhombomeres 5 and 6 of the hindbrain in both Ccm1^+/+ and Ccm1^-/- mice. (K,L) The secreted molecule shh is expressed in the notochord and ventral floor plate of the neural tube. (M,N) The homeobox gene Pax7 is expressed along the dorsal neural tube of both genotypes. (O,P) The homeobox gene Nkx2-2 is expressed in the ventral floor plate of the forebrain at E8.5. (Q,R) The axon guidance molecule Netrin1 is expressed in the ventral floor plate, as well as in adjacent somites, in Ccm1^-/- and Ccm1^+/+ mice. All markers studied showed similar expression between Ccm1^+/+ and Ccm1^-/- mice. Scale bars: 200 µm.

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