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Fig. 5. Apoptosis and proliferation analysis of Mp+/+ and
Mp–/– EBs. (A) TUNEL analysis of the
Mp+/+ and Mp–/– EBs. (a)
TUNEL staining. Fluorescent green cells are TUNEL-positive apoptotic cells.
Left panels are TUNEL staining; right panels are the overlapped images of DAPI
and TUNEL staining. Note the pattern of apoptosis in Mp+/+
(below) and Mp–/– (above) EBs. Arrows indicate
the apoptotic cells. (b) Analysis of TUNEL-positive apoptotic cells in
Mp+/+ and Mp–/– EBs. N,
the number of cells counted. No significant difference is observed between the
two classes of EBs. (B) Mp–/– EBs have a
reduced rate of cell proliferation. (a) The mitotic index of EBs was
determined by immunostaining with anti-phosphorylated histone 3 (H3P). Arrows
indicate the proliferating cells. (b) Statistical analysis of the percentage
of H3P-positive cells in Mp+/+ and
Mp–/– EBs. N, the number of cells counted.
Note that there is a significant difference between the two classes of
EBs.
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