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Fig. 6. Gain and loss of maspin function in embryoid body formation. (A-C) Rescue of defective EBs from Mp–/– ES cells using adenovirus-maspin. Note that the adenovirus control has no effect on lumen formation (arrows). (b) GATA4 staining of the adenovirus-maspin treated EBs. Arrows indicate GATA4-positive endodermal cells and arrowheads indicate the small lumen formed after adenovirus-maspin infection. Inset is Oct4 immunostaining of the adenovirus-maspin treated Mp–/– EBs. (C) Blocking of EB formation using a maspin antibody. Note that the pre-immune serum has no effect on Mp+/+ EBs. Arrows indicate the layer of cells that are detaching.





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