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Fig. 7. (A) Expression of fibronectin (a,c,e,g) and laminin (b,d,f,h) in Mp^+/+ EBs, Mp^+/+ E7.5 embryos, and Mp^–/– EBs. (a,b) Mp^+/+ EB sections after 3 days of cell culture. (c,d) Mp^+/+ EBs after 8 days of cell culture. (e,f) Mp^+/+ embryos at E7.5; (g,h) Mp^–/– EBs after 8 days of cell culture. (B) Maspin affects endodermal cell adhesion. (a) Endodermal cell adhesion to fibronectin. (b) Endodermal cell adhesion to laminin matrix. Mp^+/+ endodermal cells were treated with either preimmune serum or anti-maspin antiserum. Significant differences in cell adhesion were observed when the Mp^+/+EN, +anti-Mas and Mp^+/– EN cells to Mp^+/+ EN cells (indicated by an asterisk) were compared. (c) Decreased rate of cell proliferation in the Mp^+/– EN cells. Note the difference in the cell doubling times for the Mp^+/+ and Mp^+/– cells.

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