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Fig. 1. ERECTA-LIKE genes ERL1 and ERL2 are immediate, functional paralogs of ERECTA. (A) Amino-acid sequence alignment of ERECTA and its paralogs. The alignment was generated by the CLUSTAL X program. Identical amino acids and similar amino acids are highlighted by black and gray backgrounds, respectively. Arrows indicate the positions of introns. Respective domains are highlighted with colored underlines: N-terminal signal sequence, blue; extracellular paired cysteine regions, red; the LRR-domain, green; the transmembrane domain, black; the juxtamembrane domain, purple; the kinase domain, pink; and a C-terminal tail region, cyan. (B) A phylogenetic tree of ERECTA, ERL1 and ERL2. The single most parsimonious tree generated using heuristic searches (PAUP*) based on the kinase domain sequence is shown. BRI1 and CLV1 were used as outgroups. Branch lengths are proportional to the number of amino-acid substitutions, and the numbers at the branch points indicate bootstrap values of 500 replications. (C) ERL1 and ERL2 genes rescue the erecta phenotype when expressed under the ERECTA promoter and terminator. Shown are the inflorescence apices from wild type, erecta-105, erecta-105 plants expressing ERECTA::ERL1 and erecta-105 plants expressing ERECTA::ERL2. Scale bar: 5 mm.

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