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Fig. 6. erecta-105 erl1-2 erl2-1 triple mutations lead to dramatically
reduced plant growth, aberrant flower development, and reduced cell numbers in
petals and pedicels. (A) 12-day-old seedlings of wild type and erecta-105
erl1-2 erl2-1. Scale bars: 3 mm. (B) 24-day-old plants of
erecta-105 and erecta-105 erl1-2 erl2-1. Scale bars: 1 cm.
(C) Phenotypic variation of seven-week-old erecta-105 erl1-2 erl2-1
plants. Although the main inflorescence stem (arrowheads) always exhibits
severe elongation defects, axillary branches occasionally show various degrees
of phenotypic rescue. Scale bars: 5 mm. (D) Scanning electron micrographs of
erecta-105 and erecta-105 erl1-2 erl2-1 flowers at stage 9.
Note that one sepal was removed from the erecta-105 flower and
several sepals were removed from erecta-105 erl1-2 erl2-1. Anther
development and polar expansion of petals (arrowhead) are severely defective
in the erecta-105 erl1-2 erl2-1 flower. Scale bars: 40 µm. (E)
Flowers of erecta-105 and erecta-105 erl1-2 erl2-1 with mild
phenotype. One sepal and two petals were removed from the erecta-105
flower to expose the inner organs. Note that none of the floral organs were
removed from the erecta-105 erl1-2 erl2-1 flowers. The triple mutant
flowers have reduced numbers of organs and develop characteristic needle-like
petals. Scale bars: 0.5 mm. (F) Scanning electron micrographs of petal abaxial
epidermis cells of erecta-105 and erecta-105 erl1-2 erl2-1.
The images are taken at the same magnifications. Scale bars: 10 µm. (G)
Longitudinal sections of mature pedicels from wild type, erecta-105,
erecta-105 erl1-2, erecta-105 erl2-1 and erecta-105 erl1-2
erl2-1 plants. All combinations of mutants shown have defects in shape,
size and number of cells in the cortex cell file (asterisks). Scale bars: 50
µm. (H) Longitudinal section of the mature flower of erecta-105
and erecta-105 erl1-2 erl2-1. The lack of proper ovule and anther
(asterisk) differentiation is visible in erecta-105 erl1-2 erl2-1.
The images are taken under the same magnifications. Scale bars: 0.2 mm. (I)
Number of cells in the longitudinal cortex file of a mature pedicel of wild
type, erecta-105, erecta-105 erl1-2, erecta-105 erl2-1 and
erecta-105 erl1-2 erl2-1 plants. (J) Effect of erecta-105
and erecta-105 erl1-2 erl2-1 mutations on RNA levels of genes
regulating meristem (STM, WUS) or organ (ANT, KNAT1, D-type
cyclins) growth. Semi-quantative RT-PCR analysis was performed. An actin
fragment was amplified simultaneously as a control. The number of PCR cycles
were as follows: ANT, 28 cycles; STM, 28 cycles;
WUS, 29 cycles; KNAT1, 32 cycles; CYCD2;1, 32
cycles; CYCD3;1, 29 cycles; actin, 24 cycles.
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