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Fig. 1. The neural tube directs PNVP formation in vivo. (A) Transverse section
through the trunk of a HH stage 24 quail embryo labeled with the QH1 antibody
(brown). The arrow indicates a ring of vessels around the neural tube termed
the perineural vascular plexus (PNVP). (B) In ovo manipulations placing a
mouse neural tube (blue) between the lateral plate and intermediate mesoderm
in a quail host. (C) Section of a HH 24 stage quail embryo with an implanted
acrylic bead (asterisk) stained with QH1 antibody (purple). Note that a large
vessel is positioned near the bead (arrow), but no vascular plexus surrounds
the bead. (D) Low magnification of a transverse section through a HH stage 24
quail host with a grafted ROSA+/– mouse neural tube
(asterisk), 3 days post-surgery. Chimeric embryos were whole-mount stained for
ß-gal (blue), then sectioned and reacted with QH1 antibody (brown). (E)
Higher magnification of D. The arrow indicates a host-derived vascular plexus
surrounding the grafted neural tube. n
5 for all in ovo
manipulations. NC, notochord; NT, neural tube. Scale bar: 100 µm in A; 50
µm in C,E; 200 µm in D.
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